Disney Gender All Theatrical Animation

      All American animated theatrically released Disney films (released under Pixar Animation Studios, DisneyToon Studios, ImageMovers Digital, Skellington Productions, Walt Disney Animation, Walt Disney Television Animation), excluding films that included live action, straight to video films that had a single theater or international premier only, shorts, Disney package films, and films not produced by Disney but distributed under the Disney label. 

All Gender 1993-2012

This study looks at the gender break down of primary protagonists in family-targeting American animated films from 1993-2012. Including all G or PG-rated American (or American co-produced) animated theatrical releases that were released to at least 500 theaters and were primarily animated (there could be live action scenes, but focus was on animated characters in an animated world). Excluding package films.

151 films (percents rounded)

4 male and female co-protagonists (3%)
23 female primary protagonists (15%)
124 male primary protagonists (82%)

Disney Channel Toon Gender Ratio 1990s

From the start of 1990 to the end of 1999 there were 24 narrative cartoons that aired on Disney. Some of these started before the nineties, and some continued into the 2000s, but all cartoons that aired during this decade are included. Cartoon shows featuring shorts with a rotating cast (Raw Toonage, Mickey Mouse Works, and The Shnookums and Meat Funny Cartoon Show) were not included.

Shows:
Adventures of the Gummi Bears (1985-1991), Duck Tales (1987-1990), The New Adventures of Winnie the Pooh (1988-1991), Chip n' Dale Rescue Rangers (1989-1990), TaleSpin (1990-1991), Darkwing Duck (1991-1992), Goof Troop (1992-1993), The Little Mermaid (1992-1994), Bonkers (1993-1994), Marsupilami (1993), Aladdin (1994-1995), Gargoyles (1994-1997), The Lion King's Timon & Pumbaa (1995-1999), Quack Pack (1996), Mighty Ducks (1996-1997), Jungle Cubs (1996-1998), Disney's Doug (1996-2000), 101 Dalmatians (1997-1998), Nightmare Ned (1997), Recess (1997-2003), Pepper Ann (1997-2002), Hercules (1998-1999), PB&J Otter (1998-2000), The Weekenders (1999-2004), 

Ensemble Casts

Adventures of Gummi Bears (4M, 2F)
Tummi (M), Zummi (M), Grammi (F), Gruffi (M), Sunni (F), Cubbi (M)
Duck Tales (4M)
Scrooge McDuck (M), Huey (M), Dewey (M), Louie (M) 
Gargoyles (6M, 2F)
Goliath (M l), Elisa Maza (F), Hudson (M), Brooklyn (M), Lexington (M), Broadway (M), Angela (F), Bronx (M)
Quack Pack (4M)
Donald Duck (M), Huey (M), Dewey (M), Louie (M)
Mighty Ducks (4M 2F)
WildWing Flashblade (M l), NoseDive Flashblade (M), Duke L'Orange (M), Mallory McMallerd (F), Tanya Vanderflock (F), Check Hardwing (M),
Jungle Cubs (6M)
Baloo (M l), Bagheera (M), Louie (M), Shere Kahn (M), Kaa (M), Hathi (M)
101 Dalmations (2M, 2F)
Lucky (M l), Cadpig (F), Rolly (M), Spot (F)
Recess (4M, 2F)
T.J. (M l), Spinelli (F), Vince (M), Gretchen (F), Gus (M), Mikey (M)
PB&J Otters (2F, 1M)
Peanut (M), Butter (F), Jelly (F)
Weekenders (2M, 2F)
Tino (M l), Lor (F), Carver (M), Tish (F)

Main Character (Plus Supporting Ensemble)
The New Adventures of Winnie the Pooh (M) (6M, 1F)
Winnie the Pooh (M)
Tigger (M), Piglet (M), Rabbit (M), Eeyore (M), Owl (M), Roo (M), Kanga (F) 
TaleSpin (M) (3M, 2F)
Baloo (M)
Kit (M), Rebecca (F), Molly (F), Wildcat (M), Louis (M)
Darkwing Duck (M) (2M, 1F)
Drake Mallard (M)
Gosalyne (F), Launchpad (M), Honker (M)
The Little Mermaid (F) (2M)
Ariel (F)
Sebastian (M), Flounder (M)
Bonkers (M) (1M, 1F)
Bonkers (M)
Miranda Wright (F), Lucky Piquel (M)
Marsupilami (M) (2M)
Marsupilami (M)
Maurice (M), Stewart (M)
Aladdin (M) (1M, 1F)
Aladdin (M)
Jasmine (F), Genie (M)
Disney's Doug (M) (3M, 2F)
Doug (M)
Skeeter (M), Roger (M), Patti (F), Beebe (F), Porkchop (M)
Nightmare Ned (M)
Ned (M)
Pepper Ann (F) (3F, 1M)
Pepper Ann (F)
Nicky (F), Milo (M), Lydia (F), Moose (F)
Hercules (M) (1M, 1F)
Icarus (M), Cassandra (F)

Duo (Plus Supporting Ensemble)
Chip n' Dale Rescue Rangers (2M) (2M, 1F)
Chip (M), Dale (M)
Monterey (M), Gadget (F), Zipper (M)
Goof Troop (2M) (2M, 2F)
Goofy (M), Max (M),
Peter (M), Peg (F), P.J. (M), Pistol (F)
Timon and Pumbaa (2M)
Timon (M), Pumbaa (M)

Out of 68 main characters: 52 M, 16 F
Out of 109 main characters and main supporting characters: 78M, 31F

19 shows had a male lead and male majority (79%), 2 had a female lead and female majority, 1 had a female lead and a male majority, 2 had equal male and female main characters

In all cases where there was a leader in an ensemble cartoon (5), the leader was a male character.
In both of the cartoons that had equal male and female main characters, a male character was the leader.

While ensemble cartoons had an average of 5 characters, there were never more then 2 female characters on an ensemble show.

1/24 shows had at least 3 female main characters including ensembles (Pepper Ann with 4).
14/24 shows had 3 or more male main characters including ensembles

All Voiced Characters

Part 1: Ratio of Female to Male Characters (from 2008-2012)

All Voiced Characters with a name or character descriptor

Looking at only American, or American/Foreign Coproduced Animated Films Rated G or PG that were released in at least 500 theaters. If the film contained live action scenes, it primarily had to be about the animated characters set in an animated world*

Year: 2008 - 2012

Fact Check: Only 13 Female Protagonists

"Between 1937 and 2005 there were only 13 female protagonists in animated movies."

I stumbled upon this quote that's been floating around, originating from Miss Representation, though I can no longer find it on their site. There doesn't seem to be any source material backing it up. I feel like this is why it is so important to get some raw data out there about gender statistics in animation, because there isn't a lot to go off of at the moment.